Abstract:
Objective and Design: This study investigated the role of the prostaglandin E receptor EP3 subtype in the development of inflammatory synovitis.¶Materials and Methods: We examined the EP3 mRNA expression in the synovial tissue of rats with adjuvant arthritis and the effect of selective EP agonists on IL-6 production by cultured rat synovial cells.¶Results: Of the two rat EP3 isoforms, rEP3B mRNA but not rEP3A mRNA was expressed in rat synovial cells. RT-PCR and ELISA were used to analyze the effects of selective EP3 agonist (ONO-AE-248) on IL-6 production by rat cultured synovial cells. This selective EP3 agonist induced IL-6 production by rat synovial cells in a time- and dose-dependent manner.¶Conclusions: Our results suggest that PGE2 upregulates IL-6 production by synovial macrophages and fibroblasts through rEP3B in rats with adjuvant arthritis.¶
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Received 20 April 2000; returned for revision 29 May 2000; accepted by W. van den Berg 21 September 2000
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Kurihara, Y., Endo, H. & Kondo, H. Induction of IL-6 via the EP3 subtype of prostaglandin E receptor in rat adjuvant-arthritic synovial cells. Inflamm. res. 50, 1–5 (2001). https://doi.org/10.1007/s000110050716
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DOI: https://doi.org/10.1007/s000110050716